![]() PLV is then transported to the nucleus to activate the LacZ gene whose reporter expression is detectable as a blue color in the presence of X-gal. Interaction between the two parts of AHA1 causes cleavage release of the synthetic PLV transcription factor from Cub. Both the N-terminal moiety (aa 1–842) and the C-terminal regulatory domain (aa 838–949) were expressed as Cub-PLV and NubHA fusion protein, respectively. ( B ) Testing intramolecular interaction between the C-terminal regulatory domain and the remaining protein fragment of AHA1 using the yeast two-hybrid test based on split ubiquitin. ![]() The ost2 genes with the mutations P 68 S ( ost2-1D ) and G 867 S (in ost2-2D ), but not the wild-type AHA1, are able to complement the growth defect of RS-72. ( A ) Complementation of the yeast mutant RS-72 in which the essential gene encoding the H þ -ATPase PMA1 was placed under the strict control of a galactose (GAL)-inducible promoter. The ost2-1D and ost2-2D genes complement the yeast RS-72.
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